3D Primary Cell Culture: A New Promising Preclinical Model for Pancreatic Neuro-Endocrine Tumors (pNETs).

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Introduction: The lack of adequate in vitro and in vivo preclinical models has hampered the identification of novel treatment options and the development of personalized therapy selection for pNET patients.

Aim(s): to establish 3D culture of primary pNETs, which are viable, metabolic active and phenotypically comparable to the tumor of origin for several days after isolation.

Materials and methods: Cells are isolated from pNET specimens and cultivated for 12-15 days. Treatments start at day 3 and last until day 9-10. Growth and cell viability are monitored until the end of the experiment. Spheroids are fixed and embedded in paraffin for IHC staining on proliferation, cell death and specific neuroendocrine phenotypic markers, for comparison to the original tumor tissue.

Conference: 14th Annual ENETSConcerence (2017)

Presenting Author:

Authors: Marinoni I, Mooser C, Wiedmer T, Di Domenico A, Pantasis S,

Keywords: pNET, preclinical models, 3D culture.,