Introduction: Cell lines from hereditary medullary thyroid carcinomas (MTC) are very rare, therefore, there is a substantial need to establish new cell lines. Here, we present a novel familial MTC (FMTC) cell line, designated SCHWE.
Aim(s): The aim of this study was to establish and characterize an in vitro and in vivo model of FMTC.
Materials and methods: Tissue was obtained from a primary tumor of a 50 year old male. DNA was isolated from peripheral blood and from tumor tissue. Sequence analysis revealed a RET-mutation in Exon 13, Codon 781 (CAG>CGG) and Codon 769 (CTT>CTG). SCHWE cells were long term cultured and analyzed by microscopy, electron microscopy, immunofluorescence, FACS analysis and Array-CGH. To proof the tumorigenicity immumnodeficient NODscid gamma mice were injected with SCHWE cells.
Conference: 12th Annual ENETS Conference 2015 (2015)
Category: Basic Science - Genetics, epigenetics, miRNAs
Presenting Author: Roswitha Pfragner
, Schwach G
, Hoeger H
, Ghaffari Tabrizi-Wizsy N
, Tam Amersdorfer C
, Leitinger G
, Bock E
, Zeitlhofer P
, Haas O A
, Beichler C
, Speicher M R
, Rinner B
, Niederle B
, Strobl H
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