A Novel PKCßII Inhibitor Induces Antiproliferative Effects in Human Pancreatic Neuroendocrine Tumor Cells

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Introduction: Deregulation of the protein kinase C (PKC) signaling pathway has been implicated in tumor progression.

Aim(s): To investigate the antiproliferative and antisecretory effects of a novel PKC inhibitor (PI) in the human pancreatic endocrine cancer cell line, BON1.

Materials and methods: Cell viability was investigated by ATPlite kit, apoptosis by caspase 3/7 assay, GSK3-ß phosphorylation and Chromogranin A (CgA) secretion by ELISA, PKCßII localization by immunofluorescence, CgA expression by Western blot.

Conference: 8th Annual ENETSConcerence (2011)

Presenting Author:

Authors: Molè D, Gagliano T, Gentilin E, Bondanelli M, Tagliati F,

Keywords: PKCßII, selective inhibitor, human pancreatic neuroendocrine cell line,