A Novel Human Cell Line from Familial Medullary Thyroid Carcinoma

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Introduction: Cell lines from hereditary medullary thyroid carcinomas (MTC) are very rare, therefore, there is a substantial need to establish new cell lines. Here, we present a novel familial MTC (FMTC) cell line, designated SCHWE.

Aim(s): The aim of this study was to establish and characterize an in vitro and in vivo model of FMTC.

Materials and methods: Tissue was obtained from a primary tumor of a 50 year old male. DNA was isolated from peripheral blood and from tumor tissue. Sequence analysis revealed a RET-mutation in Exon 13, Codon 781 (CAG>CGG) and Codon 769 (CTT>CTG). SCHWE cells were long term cultured and analyzed by microscopy, electron microscopy, immunofluorescence, FACS analysis and Array-CGH. To proof the tumorigenicity immumnodeficient NODscid gamma mice were injected with SCHWE cells.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author:

Authors: Pfragner R, Schwach G, Hoeger H, Ghaffari Tabrizi-Wizsy N, Tam Amersdorfer C,

Keywords: novel MTC cell line, familial MTC, xenografts,