Cancer Stem Cells in Small Intestine Neuroendocrine Cell Line P-STS: Isolation and Molecular Characterisation


Introduction: Cancer stem cells (CSCs) represent a small subpopulation of tumour cells responsible for invasive tumour growth. Even though the presence of CSCs in neuroendocrine tumours is documented, their role in neuroendocrine tumourigenesis and metastasis is not defined as yet.

Aim(s): The aim of this study was first to characterise the SI-NET cell line P-STS regarding CSC characteristics and second, to test whether CSC capacity impacts tumourigenesis and invasive behaviour of the cells.

Materials and methods: Aldehyde dehydrogenase (ALDH) activity was detected using the Aldefluor kit. Further potential CSC markers (CD133, CD24, CD44, CD117, CD15, Nanog, Sox2, Oct4, MSI1, BMI1, ID-1 and Nestin) were analysed by flow cytometry and/or RT-PCR. A spheroid-forming assay was performed in order to simulate tumour like conditions in vitro. The invasiveness of CSCs was assessed in vivo using the chick chorioallantoic membrane (CAM) assay.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author:

Authors: Kleinegger F, Meier-Allard N, Fuchs R, Hölzl M, Krump C,

Keywords: Cancer stem cells, tumor growth, tumorigenesis & metastasis,

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