Expression of IGF/mTOR Pathway Components in Human Pheochromocytomas and In Vitro Inhibition of PC12 Rat Pheochromocytoma Cell Growth by mTOR Inhibitors
Introduction: The pathogenesis of pheochromocytomas (pheo) is poorly understood and malignant pheo need new treatment options. mTOR inhibitors, as sirolimus(S) and everolimus(E), are promising antineoplastic drugs.
Aim(s): To evaluate whether the IGF/mTOR pathways have a role in the pathogenesis and whether S and E may have antiproliferative effects in pheo.
Materials and methods: In 24 human pheo and two normal adrenal medulla (NM), we evaluated the mRNA expression of: IGFI, IGFII, IGF-I-Receptor [IGF-R], Insulin-Receptor[IR]A, IRB, IGF-IIR, IGF-Binding-Proteins [BP] 1, 2, 3 and 6, mTOR, 4EBP1 and p70S6K (by qPCR). We tested the dose- and time-dependent effects of S and E on cell growth in the PC12 rat pheo cell line.
Conference: 8th Annual ENETSConcerence (2011)
To read the full abstract, please log into your ENETS Member account.