Olfactory Receptor 51E1 is a Novel Potential Tissue Biomarker for the Diagnosis and Prognosis of Small Intestine Neuroendocrine Tumors

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Introduction: Small intestine neuroendocrine tumors (SI-NETs) get late diagnosis. We discovered the olfactory receptor 51E1 (OR51E1) as a potential target to develop as a novel biomarker.

Aim(s): To develop OR51E1 as a SI-NET novel tissue biomarker for diagnostics and prognostics.

Materials and methods: OR51E1 coding sequence was cloned from SI-NET patient specimens and NET cell lines. OR51E1 expression was analyzed by quantitative-real-time-PCR (QRT-PCR) on human normal and NET cell lines; normal liver tissue and normal hepatic cells; microdissected SI-NET cells and stroma cells. OR51E1 protein expression was analyzed by immunohistochemistry (IHC) on SI-NET slides and tissue microarray analysis (TMA). Furthermore, a new polyclonal antibody was developed.

Conference: 9th Annual ENETSConcerence (2012)

Presenting Author:

Authors: Cui T, Ryberg U, Li S, Cunningham J, Kampf C,

Keywords: small intestine neuroendocrine tumors, olfactory receptor 51E1, tissue biomarker,