Re-Cellularised Human Pancreas 3D Scaffolds as a Novel Model for Biomarker Discovery in Pancreatic Neuroendocrine Tumours (pNETs)

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Introduction: The lack of preclinical models of pNETs has challenged identification of tissue specific biomarkers of disease. Culturing cancer cells in their native tissue enables the close study of cell-ECM interaction and tissue remodelling. The biological products of such interactions can form a basis for discovery of novel biomarkers of the disease, and were previously linked with a range of inflammatory and malignant conditions.

Aim(s): To validate a decellularised human pancreas derived ECM scaffold as a novel and more clinically relevant platform for biomarker discovery and drug screening using cultured pNET cells.

Materials and methods: BON-1 and QGP-1 cell lines were cultured in decellularised pancreas derived ECM scaffolds. Viability, proliferation, and morphological characteristics were studied using viability assays and histology. Functional characterisation was performed using immunohistochemistry and genetic expression analysis with real-time PCR, and compared with 2D monolayer cultures.

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author: Ney A

Authors: Ney A, Al-Akkad W, Frenguelli L, Acedo P, Mazza G,

Keywords: 3d models, pancreatic neuroendocrine tumors, Biomarkers,