The Chemokine Receptor CXCR4 – Differential Expression in Bronchopulmonary Neuroendocrine Neoplasms

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Introduction: The CXCR4 is a plasma membrane chemokine receptor, which is involved in organogenesis, hematopoiesis and inflammation. Additionally, an over-expression is described for many neoplastic tissues. Here, an involvement in tumor progression, metastasis, adaptation to hypoxia and in stem cell survival is postulated.

Aim(s): In the present study, the expression of the CXCR4 was evaluated in a panel of neuroendocrine tumors of the lung, comprising typical carcinoids (TC), atypical carcinoids (AC) and small cell lung cancer (SCLC).

Materials and methods: CXCR4 expression was assessed by means of immunohistochemistry (IHC) using the novel rabbit anti-CXCR4 antibody UMB-2 and by qRT-PCR. The staining was quantified with three different scoring systems. Both IHC and qRT-PCR results were correlated with patient data. Additionally, endogenous CXCR4 expression was assessed in different cancer cell lines by means of immunocytochemistry.

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author: Reimann C

Authors: Reimann C, Kaemmerer D, Specht E, Wirtz R, Lupp A,

Keywords: neuroendocrine, tumor, SCLC, CXCR4, IHC,