The Chicken Chorioallantoic Membrane Assay as a Preclinical Model for the Research of Rare Gastrointenstinal Neuroendocrine Tumours

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Introduction: Preclinical trials of cancer therapeutics require both in vitro and in vivo evaluations. For neuroendocrine tumours (NET) studies are limited to cell lines, thus xenograft models are needed for screening and evaluation of anti-cancer drugs. The chick chorioallantoic membrane (CAM) assay is a well-established model system to investigate tumour growth, angiogenesis and metastasis.

Aim(s): We present CAM as a suitable model for in vivo studying of gastrointestinal neuroendocrine tumours (GI-NET).

Materials and methods: Two small intestine GI-NET cell lines (P-STS and KRJ-I) and 4 patient derived GI-NET tumour tissues were grafted on CAM for 72-120 hours at 37°C. Xenografts were observed by light microscopy and tissues were stained for NET specific markers as chromogranin A and synaptophysin. Mitogenic activity was assessed by Ki-67.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author:

Authors: Passegger C, Kump P, Haybäck J, Lipp R, Niederle B,

Keywords: Gastrointestinal neuroendocrine tumour, chicken chorioallantoic membrane assay, Patient derived tumour xenografts,