ACTG2 Inhibits Growth and Is Epigenetically Repressed in Small Intestinal Neuroendocrine Tumors

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Introduction: Small intestinal neuroendocrine tumors (SI-NETs) originate from the enterochromaffin cells in the ileum. Actin gamma smooth muscle 2 (ACTG2) is downregulated in lymph node metastases from SI-NETs. ACTG2 and microRNA-145 (miR-145) are aberrantly expressed in other cancers and ACTG2 can be induced by miRNA-145.

Aim(s): To investigate the cause for downregulation and function of ACTG2 in SI-NETs.

Materials and methods: ACTG2 protein expression was analyzed in SI-NETs (n=24) by immunohistochemistry. The SI-NET cell line CNDT2.5 was treated with 3-deazaneplanocin (DZNep), a selective EZH2 inhibitor (EPZ-6438) and a DNA hypomethylating agent (5-aza-dC). Cells were transfected with ACTG2 plasmid and miR-145. Western blotting analysis, qRT-PCR, colony-forming and viability assays were performed, and miR-145 expression levels measured in tumor samples (n=24).

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author:

Authors: Edfeldt K, Hellman P, Westin G, Stålberg P,

Keywords: SI-NET, ACTG2, miR-145, epigenetics,