Alterations of TIMP-3 Gene in Insulinomas and Its Significance

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Introduction: Our previous study on insulinoma found allelic loss of 22q12 where suppressor gene TIMP3 located. TIMP3 can inhibit tumor invasion or metastasis, but its clinical implications in insulinoma are unknown.

Aim(s): To identify molecular alterations of TIMP-3 gene in insulinomas, and correlate gene alterations with the clinicalpathological features of insulinomas.

Materials and methods: TIMP-3 protein expression was detected in 68 insulinomas and 42 paired pancreatic tissues by IHC staining. Loss of heterozygosity (LOH) was measured by using PCR combined with autoradiography in 25 insulinomas. We correlated the experimental data with clinical-pathological characteristics. P value less than 0.05 was considered significant.

Conference: 8th Annual ENETSConcerence (2011)

Presenting Author:

Authors: Chen Y, Zhou C, Wu H, Liu T, Xiao Y,

Keywords: insulinoma, TIMP-3 gene, allelic loss, biomarker,