Alternative Splicing and Differential Gene Expression in Pancreatic NETs Abstract #357
Introduction: Alternative splicing is an important mechanism to increase diversity of the proteome in higher organisms. These protein isoforms can provide as a selective drug target or marker for cancer diagnosis.
We present here a new DNA array that allows identification of splice variation and differential expression of 357 G protein-coupled receptors (GPCR) and 11 proteins of the extracellular matrix.
Aim(s): Identification of tumor specific splice variants and overexpressed genes in pancreatic NETs for targeted therapeuties and diagnostic procedures.
Materials and methods: We designed an array with 15k probes which either bind to exon, junction or intron regions. 8 pancreatic NETs and controls where hybridized.
Expression results were validated by qPCR, while splice variants where amplified by RT-PCR and sequenced.
To read results and conclusion, please login ...
Further abstracts you may be interested in
#529 Alternative Splicing and Differential Gene Expression in Pancreatic NETs
Introduction: Protein isoforms resulting from differentially spliced mRNA may constitute peculiar drug targets or markers for cancer diagnosis. We here present a new DNA array that allows identification of splice variation and differential expression for 357 G protein-coupled receptors (GPCR) and 11 proteins of the extracellular matrix.
Conference: 9th Annual ENETS Conference (2012)
Category: Basic
Presenting Author: Dr. Carsten Grötzinger
Keywords:
pNET
#1566 Splicing Dysregulation Impacts on Neuroendocrine Tumors: Evidence from Altered Spliceosoma Components and Somatostatin and Ghrelin Systems.
Introduction: Splicing is an emerging cancer hallmark influencing multiple tumor cell (dys)functions. We have shown that aberrantly spliced variants of somatostatin receptor subtype 5 (sst5TMD4) and of the pleiotropic neuropeptide ghrelin (in1ghrelin) are linked to poor prognosis in gastroenteropancreatic neuroendocrine tumors (GEPNETs) and may enhance their aggressiveness features.
Conference: 13th Annual ENETS conference (2016)
Category: Basic Science - Genetics, epigenetics, miRNAs
Presenting Author: Prof. Dr. Justo P. Castaño
Keywords:
Splicing
#930 Distinct Expression of Splicing Variants of the Human MEN1 (Multiple Endocrine Neoplasia Type 1) Gene in Various Pituitary Adenomas
Introduction: MEN1 is an autosomal-dominant tumor syndrome characterized by the occurrence of tumors in multiple endocrine tissues, including parathyroid, enteropancreatic neuroendocrine and anterior pituitary. The MEN1 gene consists of 10 exons transcribed into a 610 a.a. protein. It was reported that heterogeneity of human MEN1 gene transcripts related to variation in their 5’ UTR. Six distinct exsons1 (e1A-e1F) were isolated using RNA from thymus, pancreas and kidney.
Conference: 11th Annual ENETS Conference (2014)
Category: Basic Science - Genetics, epigenetics, miRNAs
Presenting Author: Atsushi Ozawa
#1829 Genome-Wide Analysis of Long Non-Coding RNAs in Pancreatic Neuroendocrine Tumors by Microarray
Introduction: LncRNAs have been shown to play important roles in cancer biology,but expression profile of lncRNAs in pancreatic neuroendocrine tumors(pNETs) is not well understood.
Conference: 14th Annual ENETS conference (2017)
Category: Basic Science - Genetics, epigenetics, miRNAs, Omics
Presenting Author: Meng Ji
Authors:
Ji M, ...
#1831 Genome-Wide Analysis of lncRNAs in Pancreatic Neuroendocrine Tumors by Microarray
Introduction: LncRNAs have been shown to play important roles in cancer biology,but expression profile of lncRNAs in pancreatic neuroendocrine tumors(pNETs) is not well understood.
Conference: 14th Annual ENETS conference (2017)
Category: Basic Science - Genetics, epigenetics, miRNAs, Omics
Presenting Author: Meng Ji
