Alternative Splicing and Differential Gene Expression in Pancreatic NETs

#529

Introduction: Protein isoforms resulting from differentially spliced mRNA may constitute peculiar drug targets or markers for cancer diagnosis. We here present a new DNA array that allows identification of splice variation and differential expression for 357 G protein-coupled receptors (GPCR) and 11 proteins of the extracellular matrix.

Aim(s): To identify tumor specific splice variants and overexpressed genes in pancreatic NETs.

Materials and methods: We designed an array with 15k probes which either bind to exon, junction or intron regions. Eight pancreatic NETs and eight pancreatic control tissues where hybridized. Expression results were validated by qPCR, while splice variants where amplified by RT-PCR and sequenced.

Conference: 9th Annual ENETSConcerence (2012)

Presenting Author:

Authors: Körner J, Schäfer C, Schulz P, Wiedenmann B, Grötzinger C,

Keywords: pNET,

Alternative Splicing and Differential Gene Expression in Pancreatic NETs

#357

Introduction: Alternative splicing is an important mechanism to increase diversity of the proteome in higher organisms. These protein isoforms can provide as a selective drug target or marker for cancer diagnosis. We present here a new DNA array that allows identification of splice variation and differential expression of 357 G protein-coupled receptors (GPCR) and 11 proteins of the extracellular matrix.

Aim(s): Identification of tumor specific splice variants and overexpressed genes in pancreatic NETs for targeted therapeuties and diagnostic procedures.

Materials and methods: We designed an array with 15k probes which either bind to exon, junction or intron regions. 8 pancreatic NETs and controls where hybridized. Expression results were validated by qPCR, while splice variants where amplified by RT-PCR and sequenced.

Conference:

Presenting Author:

Authors: Körner J, Wiedenmann B, Grötzinger C,

Keywords: splice variants array,