Alternative Splicing and Differential Gene Expression in Pancreatic NETs Abstract #357
Introduction: Alternative splicing is an important mechanism to increase diversity of the proteome in higher organisms. These protein isoforms can provide as a selective drug target or marker for cancer diagnosis.
We present here a new DNA array that allows identification of splice variation and differential expression of 357 G protein-coupled receptors (GPCR) and 11 proteins of the extracellular matrix.
Aim(s): Identification of tumor specific splice variants and overexpressed genes in pancreatic NETs for targeted therapeuties and diagnostic procedures.
Materials and methods: We designed an array with 15k probes which either bind to exon, junction or intron regions. 8 pancreatic NETs and controls where hybridized.
Expression results were validated by qPCR, while splice variants where amplified by RT-PCR and sequenced.
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