Introduction: Prostate cancer is the most commonly diagnosed male cancer and is a hormone-driven disease. Androgens have been recognized as a major promoter of PCa development and progression. However, the mechanism of androgen action in PCa, especially in PCa cell invasion remains largely unclear. SMAD ubiquitination regulatory factor-1 (SMURF1) is a C2-WW-HECT-domain E3 ubiquitin ligase that plays important roles in cancer cell metastasis. Whether there is a relationship between androgens and SMURF1 express is not known.
Aim(s): Identification of novel therapeutic targets for treatment of advanced PCa.
Materials and methods: The effect of androgens on the expression of SMURF1 in PCa cell lines was examined by Western blot and RT-PCR. The binding of AR to the SMURF1 gene enhancer was determined by chromatin immunoprecipitation (ChIP) assay, CHIP-Seq and RNA-Seq analysis. Cell migration and invasion was measured by wound healing and Matrigel invasion assays, respectively.
Conference: 12th Annual ENETS Conference 2015 (2015)
Category: Basic Science - Genetics, epigenetics, miRNAs
Presenting Author: Gang Xiaokun
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