Autophagy Process in Pancreatic Neuroendocrine Tumor Cells

#2030

Introduction: Pancreatic neuroendocrine malignancy is rising up in the last decade. Despite surgical resection and palliative therapy, an effective therapy has not been developed yet.

Aim(s): Here, we aim to investigate the role of autophagy and its involvement in cell death of pancreatic neuroendocrine tumor cells and stellate cells.

Materials and methods: BON1, QGP-1 (PNET cells) and HPSC 2.2 pancreatic stellate cells were treated for 24 h with 100 pM bafilomycin, an inhibitor of autophagy, and 10 nM panobinostat, an autophagy inducer. The cell growth/viability was monitored with xCelligence. RT-qPCR and Western Blot were performed to analyze autophagy markers.

Conference: 15th Annual ENETSConcerence (2018)

Presenting Author: Matrood S

Authors: Matrood S, Wissniowski T, Wiese D, Buchholz M, Gress T,

Keywords: autophagy, pancreatic neuroendocrine tumor, AMPK-alpha,