Biomarker and Target Identification in GEP-NET: From in Silico Prediction to Validation on Patient Tissues Abstract #1483

Introduction: Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are a heterogeneous group of tumors. Somatostatin agonist treatment has a key role in the management of GEP-NET. However, the prognosis of these tumors varies between individuals. There is, therefore, a need to identify novel prognostic and predictive biomarkers of therapeutic efficacy, as well as to propose novel therapeutic strategies.
Aim(s): To optimize this approach, we adopted a strategy that uses a curated biological knowledge source coupled with a proprietary data mining methodology (iFit) followed by wet lab experiments. In this poster, we highlight some of the results of the experimental validation.
Materials and methods: We selected 10 miRs and 42 mRNAs coding for G protein coupled receptors (GPCRs) that were identified with iFiT, for evaluation in tumor functioning or not from 40 metastatic or not patients (20 pancreatic NET and 20 ileal NET). The nanostring® platform was used for miRNAs and mRNAs analysis and immunohistochemistry was carried out for 11 GPCRs.
Conference: 13th Annual ENETS conference (2016)
Category: Biomarkers
Presenting Author: Pascale Plas
Keywords: GPCR, miR

To read results and conclusion, please login ...

Further abstracts you may be interested in

#1467 iFiT: An Integrative Bioinformatics Platform for Biomarker and Target Discovery. A Case Study in Neuroendocrine Tumors
Introduction: iFiT (Ipsen Focused-on-new bIological enTities and biomarkers) is a Bioinformatics platform integrating systems biology functionalities together with semantic & logic-based artificial intelligence within a high-scale computing environment.
Conference: 13th Annual ENETS conference (2016)
Category: Biomarkers
Presenting Author: PhD Sebastien Tourlet
Keywords: Targets iFiT
#624 Identification of New GPCR Targets in NET Cell Lines Using Ligand Screening
Introduction: Although somatostatin analogs constitute an important part of current therapeutic strategies, both hypersecretion and proliferation of NETs cannot be controlled efficiently in many patients. Identifying and dissecting new molecular targets and regulatory pathways may help to improve diagnostic and therapeutic options for NET patients.
Conference: 10th Annual ENETS Conference (2013)
Category: Basic Science - mTOR and other pathways, signalling, receptors
Presenting Author: Dr. Carsten Grötzinger
Authors: Körner J, Schuldt C, Giesecke Y, Du J, ...
#1465 Assocation Between miRNAs and HDACs in Pancreatic Neuroendocrine Tumors
Introduction: Epigenetic factors are essentially involved in carcinogenesis, tumor promotion and chemo resistance with their major key players of miRNAs and histone deacetylases (HDACs). As shown by own theoretical databank analysis, the crosstalk between miRNAs and HDACs are relevant in different human chronic diseases and cancerogenic pathways.
Conference: 13th Annual ENETS conference (2016)
Category: Basic Science - Genetics, epigenetics, miRNAs
Presenting Author: Prof. Dr. Daniel Neureiter
#585 Global microRNA profiling of small intestine neuroendocrine tumors (SI-NETs) and establishment of a method to study serum microRNA expression from the same tumors
Introduction: MicroRNAs (miRNAs) are important post-transcriptional regulatorsin biological processes and function either as tumor suppressors or oncogenes.
Conference:
Category: Basic
Presenting Author: Su-Chen Li
Authors: Li S C, Martijn C, Essaghir A, Lloyd R, ...
#424 Role of MicroRNAs in Small Intestine Neuroendocrine Tumors
Introduction: Small intestinal neuroendocrine tumors (SI-NETs) arise from enterochromaffin cells, which belong to the endocrine system. MicroRNAs (miRs) are post-transcriptional regulators. They control multiple biological processes often functioning either as tumor suppressor genes or oncogenes.
Conference: 9th Annual ENETS Conference (2012)
Category: Basic
Presenting Author: Su-Chen Li
Authors: Li S C, Martijn C, Essaghir A, Lloyd R, ...