Introduction: Tumour cell invasion and proliferation is a key factor for the prognosis and progression of cancer. A recent study revealed that cannabinoids can inhibit tumour invasion and proliferation in breast cancer through the down-regulation of ID-1, an inhibitor of helix-turn-helix transcription factors.
Aim(s): ID1 is highly expressed in the small intestine neuroendocrine tumour cell line P-STS. We therefore hypothesize that cannabinoids can also inhibit proliferation and invasion of P-STS via the down-regulation of ID-1.
Materials and methods: P-STS cells were treated with the CB-receptor agonist WIN-55,212. Subsequently, the ID1 expression was observed and proliferation, viability, apoptosis and mitosis were assessed. The tumour cells were xenografted onto a chicken chorioallantoic membrane (CAM) and the tissue was further stained immunohistochemically for Ki67 and a TUNEL-Assay was performed. Angiogenesis was assessed with CAM-Assays.
Conference: 13th Annual ENETS conference 2016 (2016)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: Ruben Errampalli
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