Cell Cycle Phase Expression Profile Guided Selection of Proliferative Markers for Well-differentiated Small Intestinal Neuroendocrine Neoplasms Abstract #344

Introduction: Grading of neuroendocrine lesions is often determined by Ki-67 (immunostain), despite limited prognostic ability for this cell cycle marker in well-differentiated tumors (WDNETs) and carcinomas.
Aim(s): We identified additional proliferative markers (S- and G2/M phase) in NET cell lines and compared transcript levels to Ki-67 in WDNETs and WDNECs.
Materials and methods: Microarrays of 24 small intestinal NETs (SINETs) and normals were examined (dCHIP) for over-expressed proliferation genes. Levels were determined in flow-sorted NET cell lines (propidium iodide staining/cell cycles). Proliferation biomarkers were confirmed in a second independent dataset (n=17 WDNETs (small intestine), n=21 WDNECs and 17 normals.
Conference: 8th Annual ENETS Conference (2011)
Category: Basic
Presenting Author: Dr Mark Kidd

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Keywords: biomarker, CgA, NET, PCR