Characterization of Epigenetic Modulation in Pancreatic Neuroendocrine Neoplasms

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Introduction: Pancreatic neuroendocrine tumors (PanNETs) represent a rare and heterogeneous tumor entity. Despite advances in therapy cancer progression and resistance mechanisms are still challenging. Recent genome wide sequencing analyses in PanNETs identified a large number of mutated genes involved in epigenetic modulation.

Aim(s): Targeting epigenetic regulation in tumor cells in combination with specifically triggered immune cells could be a new therapeutic avenue.

Materials and methods: The human PanNET cell lines BON-1, QGP-1, NT-3 and NT-18 the murine insulinoma cell lines beta-TC-6 and RIN-T3 and the human monocyte-like THP-1 cells were polarized in M0/M1/M2 phenotypes and treated with Panobinostat (PB) and analyzed for functional effects and affected signaling pathways performing Western blot, FACS and qPCR analyses. Additionally, the impact of PB on microRNA and lncRNA expression in BON-1 and QGP-1 cells was investigated by RNA sequencing.

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author: Schmitz R

Authors: Schmitz R, Weißbach J, Kleinlein J, Schrader J, Grabowski P,

Keywords: PanNET, HDAC-Inhibition, SOCS3, lncRNA, Macrophages,