Comparison of the Automated Chromogranin A Assay Using KRYPTOR Instrumentation with the Manual IRMA Assay of CisBio

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Introduction: Chromogranin A (CgA) has become the most important tumor marker for neuroendocrine tumors (NET) during the last decade. To meet the need for high throughput and rapid measurements, the fully automated Thermo Scientific BRAHMS KRYPTOR CgA assay has been developed.

Aim(s): To evaluate the analytical performances of the KRYPTOR assay and to compare this assay with the immunoradiometric (IRMA) assay of CisBio.

Materials and methods: The KRYPTOR assay is a sandwich immunoassay using Time Resolved Amplified Cryptate Emission technology. The incubation time is 29 minutes; measuring range is 6-1,000,000 µg/l. The CisBio assay is a solid-phase, two-site IRMA assay. Ninety-nine samples of healthy individuals and 199 patients with a NET were measured with the KRYPTOR and the CisBio assay.

Conference: 9th Annual ENETSConcerence (2012)

Presenting Author:

Authors: Korse C, Baan A, Tesselaar M,

Keywords: Chromogranin A, methods,