Comparison of the Novel Cell Detector Method with Cell Search Technology in the Isolation of Circulating Tumor Cells in Neuroendocrine Tumors

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Introduction: Circulating Tumor Cells (CTCs) have been detected in patients with neuroendocrine tumors (NET) using the CellSearch platform. Their presence is associated with a worse clinical outcome but less than 50% patients have detectable cells. The GILUPI Cell Detector is an EpCAM coated wire that is inserted into a vein and has been developed to increase capture of CTCs.

Aim(s): To compare the performance of Cell Search and the Cell Detector in patients with metastatic NETs.

Materials and methods: CTCs present in 7.5 mls blood were enumerated in NET patients by CellSearch as previously described. Concurrently, the Cell Detector wire was inserted into a vein in the anti-cubital fossa under sterile conditions for 30 mins. It was then withdrawn and analysed by immunofluorescence for cytokeratin, EpCam, DAPI and CD45. CTCs were enumerated by two operators blinded to clinical background and the CellSearch CTC count.

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author:

Authors: Mandair D, Ensell L, Vesely C, Lowe H, Caplin M,

Keywords: CTCs, EpCAM, neuroendocrine,