Cross-Talk Between EGFR and IGF1R Influences the Response to RTK Inhibitors in Bronchopulmonary (BP)-NET Cell Lines

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Introduction: BP-NETs represent ~30% of all neuroendocrine tumors. BP-NET treatment is challenging due to onset of resistance to chemo and targeted therapies. EGFR and IGF1R had been associated with tumor onset and progression in several neoplasia.

Aim(s): To investigate the role of EGFR and IGF1R and their possible cross-talk in BP-NET cells.

Materials and methods: Receptors expression was evaluated by Western blot in 2 BP-NET cell lines (NCI-H720, NCI-H727 cells). Cell viability and apoptosis were measured by ATPlite assay and Caspase3/7 assay, respectively. IGF1R and EGFR heterodimer formation was evaluated by immunoprecipitation (IP).

Conference: 13th Annual ENETSConcerence (2016)

Presenting Author: Gagliano T

Authors: Gagliano T, Gentilin E, Benfini K, Falletta S, Di Pasquale C,

Keywords: BP-NET RTK,