Introduction: The lack of relevant in-vivo and in-vitro models has precluded investigations of the pathophysiology of metastatic NENs. Organotypic tumour slices retain the complexity of tumours in-vivo without extensive manipulation of the tissue.
Aim(s): The aims of this study are to understand the utility of precision cut slice technology to develop an immunocompetent model of metastatic GEP NENs to test new therapeutics and elucidate immunological, molecular and cellular characteristics.
Materials and methods: To date, 20 participants have been recruited and 5 liver tissue samples, both tumour and surrounding ‘healthy’ liver tissue, have successfully been sliced and cultured for up to 7 days consistently and in some instances up to 15 days. Viability, metabolic activity, immunocompetency and histology have been assessed. Tissue has also been stained for Ki67 and Chromogranin A expression by immunofluorescence at all timepoints. In addition, tumour infiltrating lymphocytes, plasma, matched peripheral blood mononuclear cells, tumour culture supernatants and slice homogenates for RNA/DNA analysis have been collected and stored daily to enable further analysis.
Conference: 17th Annual ENETS Conference 2020 (2020)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: Dr Raj Srirajaskanthan
, Doornebal E
, Harris N
, Zen Y
, Pizanias M
, Prachalias A
, Zamalloa A
, Heat N
, Williams R
, Palma E
, Chokshi S
, Srirajaskanthan R
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