Establishment and Characterization of a Continuous Cell Line from a Human Familial Medullary Thyroid Carcinoma Abstract #1579

Introduction: introduction missing
Aim(s): We report the establishment of a new continuous cell line “SCHWE” from tissue that was obtained from the primary tumor of a 50 year-old male with familial medullary thyroid carcinoma (FMTC). We used isolated DNA from the tumor as well as from peripheral blood to screen for specific sequence variants in the RET proto-oncogene. We found a previously known causative mutation in exon 13, codon 781 (CAG>CGG) and also a well-known disease-irrelevant heterozygous single nucleotide polymorphism in codon 769 (CTT>CTG). CytoScan HD SNP-array (Affymetrix®) analysis revealed a widespread homozygosity as well as complex copy number abnormalities with an extraordinary multiplication of chromosome 20. Owing to an apparent ploidy increase together with variable mosaic levels made a clear-cut delineation and exact enumeration of distinct abnormalities extremely difficult. However, the combined deletion/and copy neutral homozygosity pattern of the 10q region, which contains the RET gene, suggested that one allele was lost before the other one got duplicated. Molecular genetic analysis revealed a pure wild-type pattern and therefore the loss of the two apparently linked sequence variants originally present in the tumor.
Materials and methods: materials missing
Conference: 13th Annual ENETS conference 2016 (2016)
Category: ...none of the above
Presenting Author: Roswitha Pfragner

To read results and conclusion, please login ...

Further abstracts you may be interested in

#1180 A Novel Human Cell Line from Familial Medullary Thyroid Carcinoma
Introduction: Cell lines from hereditary medullary thyroid carcinomas (MTC) are very rare, therefore, there is a substantial need to establish new cell lines. Here, we present a novel familial MTC (FMTC) cell line, designated SCHWE.
Conference: 12th Annual ENETS Conference 2015 (2015)
Category: Basic Science - Genetics, epigenetics, miRNAs
Presenting Author: Roswitha Pfragner
#1182 Epirubicin Loaded Polyphosphazene; A New Agent Against Small Intestinal Neuroendocrine Tumors and Medullary Thyroid Carcinomas
Introduction: Small intestinal neuroendocrine tumors (SI-NETs) represent a group of rare neoplasms, derived mainly from enterochromaffine cells. Medullary thyroid carcinoma (MTC) is a calcitonin-producing neuroendocrine tumor arising from the parafollicular C-cells. SI-NETs as well as MTCs are known for their poor response to standard therapy. Therefore, there is a substantial need to establish new therapeutic options in the clinical treatment of these tumors.
Conference: 12th Annual ENETS Conference 2015 (2015)
Category: ...none of the below
Presenting Author: Dr. Gert Schwach
#1528 Medullary Thyroid Carcinoma Cell Lines – An Update
Introduction: Medullary Thyroid Carcinoma (MTC) originates from calcitonin-producing neuroendocrine C-cells of the thyroid gland. Mutations in the RET-proto-oncogene are associated with both sporadic and familial MTC. As MTC are poorly responsive to chemo- and radiation- therapy, surgery is the only curative treatment at the moment. The cytogenetics of MTC have been sparsely investigated because the cells are very difficult to cultivate. In the last three decades our research group has established 10 continues cell lines derived from primary tumor and lymph node metastasis of patients with either sporadic or hereditary MTC.
Conference: 13th Annual ENETS conference 2016 (2016)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: PhD Gert Schwach
#3004 Development of Anti-SSTR CAR T Cells for Future Treatment of NETs
Introduction: NETs overexpress somatostatin receptors (SSTRs).
Conference: 17th Annual ENETS Conference 2020 (2020)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: Dr. Mauro Cives
#1107 The Chicken Chorioallantoic Membrane Assay as a Preclinical Model for the Research of Rare Gastrointenstinal Neuroendocrine Tumours
Introduction: Preclinical trials of cancer therapeutics require both in vitro and in vivo evaluations. For neuroendocrine tumours (NET) studies are limited to cell lines, thus xenograft models are needed for screening and evaluation of anti-cancer drugs. The chick chorioallantoic membrane (CAM) assay is a well-established model system to investigate tumour growth, angiogenesis and metastasis.
Conference: 12th Annual ENETS Conference 2015 (2015)
Category: ...none of the below
Presenting Author: PhD Nassim Ghaffari Tabrizi-Wizsy
Authors: Passegger C, Kump P, Haybäck J, Lipp R, ...