First In Vitro Study of Human Gastroenteropancreatic Neuroendocrine Tumors: Comparative Effect of Octreotide, Pasireotide and Everolimus

#752

Introduction: Recently pasireotide (PAS) and everolimus (RAD) emerged as potential therapies for neuroendocrine tumors (NETs).

Aim(s): To compare the effect of octreotide (OCT) PAS and RAD on cell viability, proliferation and hormonal secretion, in primary cultures of human gastroenteropancreatic-NETs (GEP-NETs).

Materials and methods: We established 13 in vitro primary cultures of GEP-NETs, using PCR and immunocytochemistry to assess expression of SSTR and MTT and BrdU assays to assess cell viability and proliferation.

Conference: 10th Annual ENETSConcerence (2013)

Presenting Author:

Authors: Albertelli M, Mohamed A, Arvigo M, Grillo F, Barbieri F,

Keywords: neuroendocrine, primary cultures, drugs,

First in vitro study of Human GastroEnteroPancreatic-Neuroendocrine Tumors: comparative effect of Octreotide, Pasireotide and Everolimus.

#587

Introduction: Recently pasireotide (PAS) and everolimus (RAD) emerged as potential therapies for neuroendocrine tumors (NETs).

Aim(s): To compare the effect of octreotide (OCT), PAS and RAD on cell viability, proliferation, hormonal secretion and growth factors modulation, in primary cultures of human gastroenteropancreatic-NETs (GEP-NETs).

Materials and methods: We established 16 in vitro primary cultures of GEP-NETs, using PCR and immunocytochemistry to assess expression of SSTR and MTT and BrdU assays to assess cell viability and proliferation.

Conference:

Presenting Author:

Authors: Albertelli M, Mohamed A, Marica A, Grillo F, Barbieri F,

Keywords: neuroendocrine, primary cultures, drugs,