Genome-Wide MicroRNA Expression in Small Intestine Neuroendocrine Tumors (“Midgut carcinoids”): Upregulation of miRNA-182, miRNA-183 and Downregulation of miRNA-215

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Introduction: MicroRNAs (miRs) may regulate cell proliferation, differentiation, apoptosis and function as tumor suppressors or oncogenes. MiR expression is not characterized in neuroendocrine midgut carcinoids.

Aim(s): Investigate miR expression of normal dissected enterochromaffin cells and tumor material to find potential involvement in tumor progression.

Materials and methods: MiR expression in patient material (five primary tumors; five mesentery and five liver metastases) and in laser-microdissected normal enterochromaffin cells and three normal ilea mucosa specimens was analyzed using miRNA Affymetrix microarray. Results were validated both with Q-RT-PCR and Northern Blot.

Conference: 8th Annual ENETSConcerence (2011)

Presenting Author:

Authors: Li S, Essaghir A, Martijn C, Lloyd R, Demoulin J,

Keywords: midgut specimens, microdissected normal enterochromaffin cells, Affymetrix, microRNA expression,