Identification of Hypoxia Induced Epigenetic Changes in the Development of Pancreatic Neuroendocrine Tumors
#969
Introduction: In VHL syndrome, pancreatic NETs develop through bi-allelic inactivation of VHL with subsequent HIF pathway activation. This also occurs in sporadic pancreatic NETs.
Aim(s): We sought to determine the epigenetic modifications occurring in VHL-related tumorigenesis in PNETs.
Materials and methods: VHL knockdown in the human pancreatic NET cell line BON1 was carried out by lentiviral transduction. Cells were cultured in normoxic and hypoxic [1% O2] conditions for 1, 4, 8, 16, 48, 72 and 168 hrs. Genome-wide methylation analysis was performed using Infinium HumanMethylation450 Arrays and gene expression analysis was performed using Illumina HT12 arrays.
Conference: 11th Annual ENETSConcerence (2014)
Presenting Author:
Authors: Dibra H, Connor T, Ashcroft M, Maxwell P, Thirlwell C,
Keywords: neuroendocrine, von Hippel-Lindau (VHL), hypoxia, epigenetics,
To read the full abstract, please log into your ENETS Member account.