Identification of Hypoxia Induced Epigenetic Changes in the Development of Pancreatic Neuroendocrine Tumors

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Introduction: In VHL syndrome, pancreatic NETs develop through bi-allelic inactivation of VHL with subsequent HIF pathway activation. This also occurs in sporadic pancreatic NETs.

Aim(s): We sought to determine the epigenetic modifications occurring in VHL-related tumorigenesis in PNETs.

Materials and methods: VHL knockdown in the human pancreatic NET cell line BON1 was carried out by lentiviral transduction. Cells were cultured in normoxic and hypoxic [1% O2] conditions for 1, 4, 8, 16, 48, 72 and 168 hrs. Genome-wide methylation analysis was performed using Infinium HumanMethylation450 Arrays and gene expression analysis was performed using Illumina HT12 arrays.

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author:

Authors: Dibra H, Connor T, Ashcroft M, Maxwell P, Thirlwell C,

Keywords: neuroendocrine, von Hippel-Lindau (VHL), hypoxia, epigenetics,

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