Increased Autophagy/Mitophagy Levels in Pancreatic Neuroendocrine Neoplasms

#2705

Introduction: Autophagy and mitophagy are key homeostatic machineries linked to cancer development and drug resistance.

Aim(s): To assess the levels of autophagy and mitophagy in well differentiated pancreatic neuroendocrine neoplasms (PanNENs) and correlate them with clinico-pathological parameters.

Materials and methods: Fluorescent immunostaining for the autophagy markers LC3Β and p62/or LAMP1 was performed on 22 PanNENs and 11 controls of normal pancreatic tissues and validated through Western blotting. Autophagy quantitative scoring was generated for LC3B-positive puncta and analyzed in relation to clinico-pathological parameters. TOMM20/LC3B qualitative assessment of mitophagy levels was undertaken by fluorescent immunostaining. The presence of autophagy/mitophagy was validated by transmission electron microscopy.

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author:

Authors: Daskalakis K, Alexandraki K, Kloukina I, Kassi E, Felekouras E,

Keywords: Pancreatic Neuroendocrine Tumors, Autophagy, Mitophagy,

To read the full abstract, please log into your ENETS Member account.