Influence of VEGF Splicing on Microvessel Density and Architecture in Pancreatic Neuroendocrine Tumours Abstract #1850

Introduction: Hypoxia in human tumours is associated with a poor prognosis and is characterized by the stabilization of HIF1α, upregulation of VEGF, and a high microvessel density (MVD). Interestingly, although pancreatic neuroendocrine tumours (pNETS) showing hypoxia signalling also have a poor prognosis, they paradoxically display a low MVD. We hypothesized that this paradoxon might be explained by alternative splicing of VEGF.
Aim(s): Our aim was to analyze the microvessel architecture in pNETs with high and low MVD and to investigate the role of VEGF-A splicing in these tumours as well as in the human pNET cell lines Bon1 and QGP1.
Materials and methods: In each five pNETs with high and low MVD microvessel architecture was examined using 3D reconstruction. VEGF-A splicing was examined in the same tumours as well as in Bon1 and QGP1 under normoxic and hypoxic conditions.
Conference: 14th Annual ENETS conference 2017 (2017)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: MD Anja Maria Schmitt
Keywords: pNET, VEGF

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