Introduction: miRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs and their expression is often dysregulated in cancer.
Aim(s): Little is known about the carcinogenesis of the slow growing neuroendocrine tumors and we therefore wanted to characterize miRNA expression in these.
Materials and methods: miRNA expression in carcinoids, metastasis and normal intestinal tissue was characterized using miRNA arrays. Changes in miRNA expression were validated by in-situ hybridization and qPCR. miR-129-5p and let-7 was selected and further characterized by mRNA arrays on total RNA from cells transfected with either miRNA. Heatmaps identified let-7 target genes involved in metastasis. miR-129-5p and its two targets EGR1 and G3BP1 were validated by western blot analysis and luciferase assay and further examined by growth assays with cells transfected with miR-129-5p or siRNAs against EGR1 and G3BP1.
Conference: 9th Annual ENETS Conference (2012)
Presenting Author: Kristina Døssing
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