Molecular Profiling of Small Intestinal Neuroendocrine Tumours (SINETs)

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Introduction: Aberrant DNA methylation plays an role in the pathogenesis of many cancers, however little is known about its role in SINET development.

Aim(s): We report the first unbiased genome-wide DNA methylation analysis of a large cohort of SINET, aiming to identify epimutations specific to SINET which may contribute to tumorigenesis.

Materials and methods: HumanMethylation450 Array analysis was performed on DNA from SINET tumours (n=49) and normal SI (n=21). Publicly available methylation data on >600 intestinal adenocarcinoma was also assessed for comparison. Gene expression was determined using the DASL array on RNA from SI NET tumours (n=32) and normal SI (n=6). Analysis was performed using ChAMP and limma R packages. A Bonferroni adjusted significance threshold of p<0.05 was used throughout.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author: Thirlwell C

Authors: Karpathakis A, Feber A, Morris T, Dibra H, Pipinikas C,

Keywords: Epigenetics, methylation, GIPR, biomarker,