Orexins Exert a Pro-Apoptotic Effect on Neuroendocrine Tumors (NETs) in an Ex Vivo Culture Model of Tissue Slices

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Introduction: Ex vivo culture models of tissue slices are suitable to test the effect of antitumor agents in human tumors. The orexins are neuropeptides binding to G protein-coupled receptors (OX1-R and OX2-R), exerting a pro-apoptotic effect in human colon and pancreatic cancer cell lines.

Aim(s): Development of an ex vivo culture model of human digestive NET, evaluation of tumor apoptotic response to orexin-A.

Materials and methods: After taking a core biopsy of the tumor (from surgical specimens), sections of 300 microns were made using a "tissue slicer" and cultured in a suitable medium at 37°C for 72 hours with and without addition of orexin-A (10-4M). Proliferation, apoptosis, differentiation and the presence of orexin receptors were studied by histology and immunohistochemistry (Chromogranin A, Ki-67, caspase-3, OX1-R).

Conference: 9th Annual ENETSConcerence (2012)

Presenting Author:

Authors: Palazzo M, Rebours V, Voisin T, Albuquerque M, Paradis V,

Keywords: digestive NET, orexins, apoptosis, ex vivo model,