PCR Assessment of Carcinoid Lymph Node Metastasis CgA Transcript Outperforms Immunostaining, Tyramide Signal Amplification and H&E and Significantly Upstages Disease

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Introduction: Accurate neuroendocrine tumor (NETs) staging is vital for determining therapeutic strategy. Sensitive techniques to facilitate identification of metastases (mets) are of critical clinical relevance.

Aim(s): To compare PCR for CgA with immunofluorescent (IMF) staining, tyramide signal amplification (TSA) and H&E for lymph node (LN) met detection.

Materials and methods: We evaluated LNs (n=22) from SINET resections (n=7). Contiguous sections (n=3) were prepared with H&E, CgA and DAPI (IF), or CgA and DAPI augmented by TSA. Pathologists (n=3) independently examined de-identified slides. IF and TSA positivity required nuclear involvement (DAPI localized). Lastly, we performed QPCR of lymph node mRNA for CgA (n=22).

Conference: 8th Annual ENETSConcerence (2011)

Presenting Author:

Authors: Lawrence B, Kenney B, Kidd M, Modlin I,

Keywords: CgA, immunofluorescence, histopathology, lymph node, neuroendocrine tumor, PCR, tyramide, up-staging,