Prolonged cell survival in xenografts from human digestive endocrine tumors Abstract #113

Introduction: Gastroenteropancreatic endocrine tumors have the capacity to achieve very large tumor masses despite usually very low proliferative rates. This suggests that neoplastic endocrine cells may have long life spans, implying the development of specific mechanisms able to promote cell survival.
Aim(s): In a first attempt to test this hypothesis, we decided to study the duration of survival of neoplastic endocrine cells obtained from fresh human tumor tissue and transplanted into the nude mouse.
Materials and methods: Fresh tissues were obtained from eight tumors (six primary, two metastatic) obtained from seven patients enrolled after informed consent. There were two pancreatic tumors (one glucagon-positive, one gastrinoma); four were of intestinal origin (all were serotonin positive; one was associated with the carcinoid syndrome); one was a mesenteric lymph node metastasis from a primary tumor located in a Meckel diverticulum. The glucagon-positive pancreatic tumor was classified as a well-differentiated tumor of benign behavior; all other cases were well-differentiated endocrine carcinomas. Immediately after surgical resection, small pieces (2x2x2 mm) of fresh tumor tissue were transplanted subcutaneously between the scapulae under anaesthesia into 4- to 5-week-old female Balb/c nude mice (eight mice per tumor). Animals were sacrificed one, two, four and six months after grafting; tumor tissues were either fixed in buffered formalin or frozen in liquid nitrogen for further histological and immunohistochemical techniques. When possible, tumor samples derived from grafted tumors were retransplanted.
Conference: 7th Annual ENETS Conference (2010)
Category: Basic
Presenting Author: Colette Roche

To read results and conclusion, please login ...

Further abstracts you may be interested in

#308 Goblet Cell Carcinoid Tumors - A Retrospective Study of Clinical Presentation and Survival
Introduction: Goblet cell carcinoids (GCC) are rare mixed tumors, with partial neuroendocrine differentiation and intestinal-type goblet cell morphology. They usually have a highly malignant potential.
Conference: 8th Annual ENETS Conference (2011)
Category: Clinical
Presenting Author: Dr Henning Grønbæk
Authors: Holt N, Villadsen G, Krogh K, Olesen R, ...
#536 Orexins Exert a Pro-Apoptotic Effect on Neuroendocrine Tumors (NETs) in an Ex Vivo Culture Model of Tissue Slices
Introduction: Ex vivo culture models of tissue slices are suitable to test the effect of antitumor agents in human tumors. The orexins are neuropeptides binding to G protein-coupled receptors (OX1-R and OX2-R), exerting a pro-apoptotic effect in human colon and pancreatic cancer cell lines.
Conference: 9th Annual ENETS Conference (2012)
Category: Basic
Presenting Author: Dr Maxime Palazzo
#602 Antitumoral Effect of Everolimus in Preclinical Models of Poorly Differentiated Neuroendocrine Carcinomas
Introduction: For the small and large cells poorly differentiated neuroendocrine carcinomas (PDNECs) emergency treatment remains the combination cisplatin/etoposide. However, recent studies suggest that targeted therapies could be a therapeutic option for this group of highly aggressive carcinomas.
Conference: 10th Annual ENETS Conference (2013)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: Colette Roche
Authors: Bollard J, Couderc C, Blanc M, Poncet G, ...
#920 High Grade (G3) Neuroendocrine Neoplasms Should Be Further Classified According to Morphological Differentiation
Introduction: Neuroendocrine neoplasms (NENs) with a Ki-67 >20% (Grade 3) are classified together as Neuroendocrine Carcinomas (NEC), and are usually considered as poorly differentiated. However Grade (G)3 NENs may be heterogeneous, with some demonstrating a well-differentiated cell morphology.
Conference: 11th Annual ENETS Conference (2014)
Category: ...none of the below
Presenting Author: Dr Dalvinder Mandair
Keywords: Ki-67, g3 NENs
#1096 How Manage Multiple Small Bowel Neuroendocrine Tumors?
Introduction: Multiple small bowel neuroendocrine tumors (SB-NET) represent 30% of SB-NET.
Conference: 12th Annual ENETS Conference (2015)
Category: Surgical treatment
Presenting Author: MD, PhD Thomas Walter
Close
Notice

WARNING – FRAUDULENT WEBSITES

ENETS is aware that its members have been confronted with fake websites, which seem to relate to our congress but have not been commissioned or authorised by us. The websites offer fraudulent hotel reservation services. We therefore strongly recommend that you please only use the official enetsconference.org website when booking your tickets and accommodation. Please be vigilant when it comes to providing your credit card details. 

If you have any questions, please do not hesitate to contact us at info@enets.org.