Promoter Hypermethylation at RASSF1 is a Feature of Small Intestinal Neuroendocrine Tumors

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Introduction: Promoter hypermethylation of RASSF1 has been reported in many tumor types.

Aim(s): Unbiased molecular analysis of small intestinal neuroendocrine tumors (SI NETs) has been performed which identified significant differential methylation of RASSF1 between SI NETs and normal tissue.

Materials and methods: Methylation analysis (Infinium HumanMethylation450 Array) was performed on 94 samples from 56 cases; gene expression analysis (HumanWholeGenome DASL Array) was performed on 64 samples from 55 cases; plasma circulating free DNA (cfDNA) hypermethylated RASSF1 detection (methylation sensitive restriction enzyme digestion and RT-PCR) was performed on 12 samples.

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author: Karpathakis A

Authors: Karpathakis A, Dibra H, Morris T, Feber A, Mandair D,

Keywords: Epigenetics, methylation, RASSF1,

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