Promoter Hypermethylation at RASSF1 is a Feature of Small Intestinal Neuroendocrine Tumors Abstract #936

Introduction: Promoter hypermethylation of RASSF1 has been reported in many tumor types.
Aim(s): Unbiased molecular analysis of small intestinal neuroendocrine tumors (SI NETs) has been performed which identified significant differential methylation of RASSF1 between SI NETs and normal tissue.
Materials and methods: Methylation analysis (Infinium HumanMethylation450 Array) was performed on 94 samples from 56 cases; gene expression analysis (HumanWholeGenome DASL Array) was performed on 64 samples from 55 cases; plasma circulating free DNA (cfDNA) hypermethylated RASSF1 detection (methylation sensitive restriction enzyme digestion and RT-PCR) was performed on 12 samples.
Conference: 11th Annual ENETS Conference (2014)
Category: Basic Science - Genetics, epigenetics, miRNAs
Presenting Author: Dr Anna Karpathakis

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