Quantification of Somatostatin Receptor Subtypes Using In Vivo PET/CT-Data, Ex Vivo Conventional Immunohistochemistry and Automated Digitized Analysis by Definiens XD Image Software Abstract #791

Introduction: Diagnostics and therapy of NET are significantly influenced by their somatostatin receptor (SSTR) status. Immunohistochemistry (IHC) of SSTR performed by pathologists is a time-consuming and cost-intensive procedure.
Aim(s): To evaluate the comparability of an automated and conventionally histopathologically examined SSTR expression.
Materials and methods: We examined 25 NET-patients and correlated their in vivo SSTR-PET/CT data (SUVmax, SUVmean) with the corresponding ex vivo-immunohistochemical (IHC) data of SSTR (1, 2A, 4, 5) expression. Exactly the same lesions were imaged by PET/CT, resected and analyzed by IHC in each patient. The IHC slides were then digitized and automatically evaluated for SSTR expression by Definiens Tissue Studio software. A virtual IHC score “BB1” was created for comparing the manual and automated analysis of SSTR expression.
Conference: 11th Annual ENETS Conference (2014)
Category: Pathology, grading, staging
Presenting Author: Dr. med. Daniel Kaemmerer
Keywords: SSTR

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