Role of Amplifications in the PIK3/Akt/mTOR-Pathway in Neuroendocrine Tumors of the Small Intestine

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Introduction: Amplifications in the PIK3/Akt/mTOR pathway can lead to increased proliferation and reduced apoptosis, resulting in the development of various cancers. Recently, amplifications of Akt1/2, PDGFRα/β and mTOR in small intestine neuroendocrine tumors (siNET) have been shown.

Aim(s): We sought to identify the distribution and biological relevance of amplifications of Akt1/2, PDGFRα/β and mTOR in small intestine neuroendocrine tumors analyzing a large cohort.

Materials and methods: Gene amplifications were assessed by Fluorescence-in-situ-Hybridization (FISH). Tissue microarrays consisting of 217 siNET samples of 135 different patients were used. Amplification was determined by the ratio of Akt1/2, PDGFRα/β, mTOR specific signals and of reference chromosome signals. Samples showing >2 signals in >30% of cells were referred to as amplified.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author:

Authors: Hoffmeister M, Sperveslage J, Anlauf M, Petersen K, Gress T,

Keywords: NET, gene amplification, mTOR pathway,