Role of Epithelial-to-Mesenchymal Transition Markers in Predicting Bone and Visceral Metastases from NETs Abstract #908

Introduction: Epithelial-to-mesenchymal transition (EMT) of tumor cells has been recently postulated as a pivotal mechanism driving metastatic spread.
Aim(s): A tumor-specific gene signature may be useful to predict the susceptibility of neuroendocrine tumors (NETs) to metastasize toward the skeleton.
Materials and methods: Twenty-one NETs were grouped in two arms based on the presence or absence of metastases. Tumor sections were immunostained to molecules involved in metastatic homing (CXCR4, RANK) and EMT (TGFβ1, SNAIL, CTGF, PTHrP, IL11) and the data interpreted by Allred score. H727, H720, H835, CM, QGP1 and BON1 cells were screened with RT-PCR for genes enrolled in osteoclastogenesis (RANK, TNFα, PTHrP, TRAcP, vATPase, MMP9, MMP13) and in osteoblastogenesis (Osteocalcin, Osterix, RUNX2, COL1A1, ALP).
Conference: 11th Annual ENETS Conference 2014 (2014)
Category: Biomarkers
Presenting Author: Valeria Simone
Keywords: EMT, biomarker

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