Role of TSC22D1 (TGFβ-Stimulated Clone 22 Domain Family Member 1) in Bronchial Carcinoids Abstract #1301

Introduction: Neuroendocrine tumors (NETs) include bronchial carcinoids, either typical (TC) or atypical (AC). Tsc22d1 encodes for a member of TSC22 domain family of leucine zipper transcription factors; the protein (TSC22D1) is stimulated by TGFβ. Microarray data analysis obtained comparing a pool of TC tissue specimens with a pool of AC tissue specimens shows TSC22D1 down-regulation in AC samples. These data were confirmed by real time PCR and Western blot in vitro models of TC (NCI-H727 cells) and AC (NCI-H720 cells)
Aim(s): To evaluate the influence of TSC22D1 on cell proliferation, cell viability and apoptosis in NCI-H727 cell line
Materials and methods: TSC22D1 was silenced by shRNA in NCI-H727 cell line to obtain a stable clone (shTSC22D1-NCI-H727). NCI-H727 and shTSC22D1-NCI-H727 cells were treated with TGFβ. Cell viability, caspase activation and cell proliferation were evaluated in both cell lines
Conference: 13th Annual ENETS conference (2016)
Category: Biomarkers
Presenting Author: PhD student Simona Falletta

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