The Assessment of Proliferation in BP-NEN Entities Based on the Three Proliferation Markers Ki-67, TOP2A and RacGAP1

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Introduction: The extent of cell proliferation as an important component of carcinogenesis can be detected in tumor tissues by means of various factors.

Aim(s): We examined whether the proliferation markers Ki-67, TOP2A and racGAP1 vary in bronchopulmonary neuroendocrine neoplasms (BP-NEN), comprising typical carcinoids (TC), atypical carcinoids (AC), small cell lung cancer (SCLC) and large cell neuroendocrine carcinomas (LCNEC).

Materials and methods: In 92 formalin-fixed, paraffin-embedded tumor or metastases samples (26 TC, 30 AC, 35 SCLC, one LCNEC), immunohistochemistry (IHC) was performed and evaluated semiquantitatively. Adjacent paraffin sections from 20 randomly selected TC, AC and SCLC underwent qRT-PCR analysis.

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author:

Authors: Specht E, Wirtz R, Kaemmerer D, Schulz S, Sänger J,

Keywords: Ki-67, TOP2A, racGAP1, pulmonary, NEN, RT-PCR, immunohistochemistry,