The Neuroendocrine Phenotype, Genomic Profile, and Therapeutic Sensitivity of GEPNET Cell Lines Abstract #2196

Introduction: Patient tumour-derived cell lines have been widely used for studying the molecular mechanisms of tumours and their response to therapy. The establishment of cell lines from gastro-entero-pancreatic neuroendocrine tumours (GEPNETs) has proved difficult, but despite the challenges a limited amount of cell lines now exists.
Aim(s): In this study, we aimed to define the neuroendocrine phenotype, molecular characteristics, and the therapeutic sensitivity of frequently used GEPNET cell lines.
Materials and methods: Immunophenotyping, copy-number profiling, whole-exome sequencing, and an inhibitor screening with over 1,200 inhibitors was performed on seven GEP-NET cell lines. Furthermore, primary cells were established from patient GEPNETs collected at surgery to evaluate the effect of MEKi and HDACi.
Conference: 15th Annual ENETS conference (2018)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: Tobias Hofving

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