Zebrafish as a New In Vivo Model to Study Angiogenesis in Neuroendocrine Tumors (NET)

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Introduction: Zebrafish is a powerful model to study human diseases, including cancer.

Aim(s): A novel in vivo angiogenesis assay based on the injection of human NET cell lines (TT, MZ-CRC1, DMS79 and PC3) and primary NET cell cultures has been developed in Tg(fli1:EGFP)y1 zebrafish embryos, in which the fluorescent protein EGFP is expressed in the vascular tree.

Materials and methods: NET cells were stained with red fluorescent dye and injected in the proximity of the developing sub intestinal vessel (SIV) plexus in Tg(fli1:EGFP)y1 embryos. The vasoproliferative response has been detected by both fluorescence microscopy and alkaline phosphatase staining at 24–48 hours post injection.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author: Vitale G

Authors: Gaudenzi G, Dicitore A, Cotelli F, Ferone D, Persani L,

Keywords: zebrafish, NET,