Introduction: Zebrafish is a powerful model to study human diseases, including cancer.
Aim(s): A novel in vivo angiogenesis assay based on the injection of human NET cell lines (TT, MZ-CRC1, DMS79 and PC3) and primary NET cell cultures has been developed in Tg(fli1:EGFP)y1 zebrafish embryos, in which the fluorescent protein EGFP is expressed in the vascular tree.
Materials and methods: NET cells were stained with red fluorescent dye and injected in the proximity of the developing sub intestinal vessel (SIV) plexus in Tg(fli1:EGFP)y1 embryos. The vasoproliferative response has been detected by both fluorescence microscopy and alkaline phosphatase staining at 24–48 hours post injection.
Conference: 12th Annual ENETS Conference 2015 (2015)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: Dr Giovanni Vitale
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