Effects of Multi-Receptor Targeting Drugs in Neuroendocrine Tumors Using 3D Cell Culture Abstract #2271

Introduction: Symptom control in functioning neuroendocrine tumors(NETs) may be difficult in some cases, thus, new therapeutic options, including multi-receptor targeting drugs are required.
Aim(s): Since new in vitro tumor models seem to better mimic in vivo conditions, we aimed to study the effect of somatostatin (sst) and dopamine (D2R) receptor agonists (octreotide-OCT, cabergoline-CAB, respectively) and sst-D2R multi-receptor targeting drugs (BIM065,BIM23A760) using 2D and 3D (spheroids) cultures.
Materials and methods: Human BON-1 and QGP-1 pancreatic NET cells were used. Dose-response studies in 2D and 3D cultures were performed in medium with 10% FBS and 0.1% BSA. Total cell number, sst and D2R expression by qPCR, and serotonin and CgA secretion were assessed.
Conference: 15th Annual ENETS conference (2018)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: Aura Dulcinea Herrera-Martínez

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