Effects of Multi-Receptor Targeting Drugs in Neuroendocrine Tumors Using 3D Cell Culture

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Introduction: Symptom control in functioning neuroendocrine tumors(NETs) may be difficult in some cases, thus, new therapeutic options, including multi-receptor targeting drugs are required.

Aim(s): Since new in vitro tumor models seem to better mimic in vivo conditions, we aimed to study the effect of somatostatin (sst) and dopamine (D2R) receptor agonists (octreotide-OCT, cabergoline-CAB, respectively) and sst-D2R multi-receptor targeting drugs (BIM065,BIM23A760) using 2D and 3D (spheroids) cultures.

Materials and methods: Human BON-1 and QGP-1 pancreatic NET cells were used. Dose-response studies in 2D and 3D cultures were performed in medium with 10% FBS and 0.1% BSA. Total cell number, sst and D2R expression by qPCR, and serotonin and CgA secretion were assessed.

Conference: 15th Annual ENETSConcerence (2018)

Presenting Author: Herrera-Martínez A

Authors: Herrera-Martínez A, Van Dungen R, Van Koetsveld P, Dogan-Oruc F, Culler M,

Keywords: NETs, somatostatin receptors, dopamine receptors, multi-receptor targeting drugs, hormone secretion,