Epigenetic Modulation of Autophagy in Pancreatic Spheroid Cells Abstract #2073

Introduction: Pancreatic neuroendocrine malignancy is rising up in the last decade. Despite surgical resection and palliative therapy, an effective therapy has not been developed yet.
Aim(s): Here, we aim to investigate the role of autophagy mediated by epigenetic modifications in spheroids of BON1 and stellate cells.
Materials and methods: BON1 and HPSC 2.2 pancreatic stellate cells spheroids were stably transfected with MAP1LC3B-GFP-RFP and treated up to 72h with 100 pM bafilomycin, an inhibitor of autophagy, and 10 nM panobinostat, an autophagy inducer. RT-qPCR, Western Blot and immunofluorescence were performed to analyze autophagy markers.
Conference: 15th Annual ENETS conference 2018 (2018)
Category: Basic Science - In vitro models, tumor growth, CTCs
Presenting Author: PhD Pietro Di Fazio

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