Epigenetic Modulation of Autophagy in Pancreatic Spheroid Cells

#2073

Introduction: Pancreatic neuroendocrine malignancy is rising up in the last decade. Despite surgical resection and palliative therapy, an effective therapy has not been developed yet.

Aim(s): Here, we aim to investigate the role of autophagy mediated by epigenetic modifications in spheroids of BON1 and stellate cells.

Materials and methods: BON1 and HPSC 2.2 pancreatic stellate cells spheroids were stably transfected with MAP1LC3B-GFP-RFP and treated up to 72h with 100 pM bafilomycin, an inhibitor of autophagy, and 10 nM panobinostat, an autophagy inducer. RT-qPCR, Western Blot and immunofluorescence were performed to analyze autophagy markers.

Conference: 15th Annual ENETSConcerence (2018)

Presenting Author: Matrood S

Authors: Matrood S, Wissniowski T, Wiese D, Gress T, Bartsch D,

Keywords: spheroid, autophagy, pancreatic neuroendocrine tumor, AMPK-alpha, stellate cells,