Functional Role of miR-196a in Neuroendocrine Tumor Cells

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Introduction: An exclusive miRNA profiling of small intestinal neuroendocrine tumors (SI-NETs) was published in 2013; and nine miRNAs were selected for further investigations. We chose miR-196a to elucidate its target genes and biological functions, by using NET cells as in vitro models.

Aim(s): To identify miR-196a target genes and explore their biological functions.

Materials and methods: MiR-196a targets prediction analysis suggested four target genes for further investigation. We used anti-miR-196a to silence CNDT2.5 and NCI-H727 cells. Target genes expression and encoded proteins were analyzed by quantitative real-time PCR and western blot analyses. Furthermore, migration assay was performed.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author:

Authors: Shi H, Li S, Khan M, Caplin M, Meyer T,

Keywords: neuroendocrine tumors, miRNA target genes, miRNA silencing,