Investigating the Crosstalk between MEN1, p53 and Notch Reveals Biomarkers of Formation of Primary Pancreatic Neuroendocrine Tumors Abstract #2707

Introduction: Pancreatic neuroendocrine tumors (PNETs) comprise a heterogeneous group of endocrine tumors. Previous studies showed that mutations of p53 are extremely rare; whereas genetic changes of MEN1 frequently occur and are correlated with poor prognosis in PNET. Still, their roles in tumorigenesis remain elusive.
Aim(s): The aim was to understand the mechanisms behind PNET tumorigenesis and develop a diagnostic method based on the combination of previously identified genetic abnormality with novel molecular markers.
Materials and methods: Using MEN1 mouse model, PNET human tissues and human cell lines with wild-type p53 (NT3) and mutated p53 (BON1 and QGP1), we studied interactions between MEN1 and p53 in PNET tumorigenesis. Tissues were analyzed using immunohistochemistry and cell lines were subjected to qPCR, flow cytometry, immunofluorescence, western blotting and proliferation assay.
Conference: 17th Annual ENETS Conference 2020 (2020)
Category: Basic Science - Signaling pathways, receptors, biomarkers
Presenting Author: Dr Ylenia Capodanno

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