MicroRNA Expression in Serum of Small Intestine Neuroendocrine Tumor Patients and miR-196a Biological Function in Neuroendocrine Tumor Cells

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Introduction: We have previously identified five upregulated and four downregulated miRNAs in small intestinal neuroendocrine tumor (SI-NET) tissue. We extended our analysis to serum samples of SI-NET patients to depict miRNAs functions.

Aim(s): 1) To analyze the expression of nine miRNAs in serum from SI-NET patients. 2) To explore the biological functions of their potential miRNA target genes.

Materials and methods: Total RNA of serum samples from 21 SI-NET patients at different stage of disease, and seven healthy donors was used to study miRNAs expression by using QRT-PCR analysis. Functions of miRNAs were investigated by using QRT-PCR and NET cells as in vitro models.

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author: Li S

Authors: Li S, Khan M, Caplin M, Öberg K, Meyer T,

Keywords: neuroendocrine tumors, serum miRNA expression, miRNA, target functions ,