Osteotropism of NETs: Role of the SDF1/CXCR4 Axis in Regulating EMT
#1520
Introduction: NETs metastasize to the bone in up to 20% of patients. The SDF1/CXCR4 axis is a key determinant of osteotropism, and we showed that NETs overexpressing CXCR4 are more likely to develop bone metastases.
Aim(s): To explore the biologic mechanisms underlying CXCR4-driven NET bone metastases.
Materials and methods: BON1, QGP1, CM, H727 and CNDT2.5 cells were evaluated in their CXCR4 expression by flow cytometry. Migration and invasion towards the bone or SDF1-conditioned medium were assessed by functional assays. Transcription and expression levels of EMT-related genes were assessed by RT-PCR and immunocytochemistry before and after SDF1 treatment. Secretion of SDF1 was studied by ELISA. Confocal microscopy and WB were used to investigate the subcellular distribution of CXCR4.
Conference: 13th Annual ENETSConcerence (2016)
Presenting Author: Cives M
Authors: Simone V, Quaresmini D, D'Oronzo S, Rizzo F, Felici C,
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