Valproic acid induces apoptosis-mediated cell death of the ileal carcinoid cell line GOT1

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Introduction: Downregulation of tumor suppressor genes, evasion from apoptosis, and reduced differentiation are hallmarks of cancer that can be due to abnormal epigenetic control. Histone deacetylase (HDAC) is an enzyme involved in the deacetylation of histone proteins, resulting in chromatin remodelling and altered gene transcription. HDAC inhibitors are emerging as useful anticancer drugs.

Aim(s): To investigate the effects of the HDAC inhibitor valproic acid (VPA) on growth and biological responses in human carcinoid cells.

Materials and methods: Cell proliferation, cell cycle analysis and activation of apoptosis were analyzed in an ileal (GOT1) and a pancreatic (BON) carcinoid cell-line that had been treated with 0-4mM VPA. To further determine the apoptosis pathways involved in the VPA-induced cell death of GOT1 cells, gene expression profiles were generated of GOT1 cells treated with 4 mM VPA for 28 hours.

Conference: 7th Annual ENETSConcerence (2010)

Presenting Author:

Authors: Arvidsson Y, Ahlman H, Nilsson O,

Keywords: carcinoid, HDAC, apoptosis, valproic acid,